Plasmid_Backbone

Part:BBa_K4841017:Design

Designed by: Qiu Zexuan   Group: iGEM23_TSBC-SZ   (2023-08-12)


pCOLA-PesaS-RBS-RFP-rrnbT1-CI857-PR-SRRz-rrnB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 4111
    Illegal NheI site found at 5340
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 206
    Illegal BamHI site found at 954
    Illegal XhoI site found at 2154
    Illegal XhoI site found at 4006
    Illegal XhoI site found at 4117
    Illegal XhoI site found at 5989
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 4325


Design Notes

no


Source

no

References